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z BD LSRFortessa Cell Analyzer Site Preparation Guide: FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day. There is no fluidics startup for the Fortessa. BD High Throughput Sampler User’s Guide For the BD LSRFortessa I. Introduction The BD High Throughput Sampler (HTS) is a high-speed sampling device used on the 5-Laser LSRFortessa located in Rm. 6006F of the RIA Division. The HTS provides users the ability to acquire samples from 96-well or 384-well plates for efficient analysis with Flow Cytometry Service BD LSR Fortessa SORP – How to Use Guide 5 4 Create a New Specimen (click the icon with a syringe). 5 Select Tube_001 (inside the syringe) by clicking in the green arrow. 6 Do 3 PRIMEs with the SIP 2 arm open and no tube installed. Then set the cytometer to RUN. 7 Acquire and record dH 2O for 3 minutes in HIGH (name it ‘Initial’).

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BD LSR Fortessa Flow Cytometry Analyzer equipped with HTS (High Throughput Sampler) The facility at 2117A Everitt Lab (click link for campus map) is equipped with a BD LSR Fortessa analyser. It is very similar in operation to the LSR II cytometers (RAL and ERML Satelite Facilities) but with greater analysing sensitivity. BD LSR Fortessa (16 colour Analyser) 4 lasers, 16 filters (see table below). Sample loading via tubes. Table of lasers and filters available: LSR Fortessa manual; The Danish Stem Cell Center (DanStem) Appendix’B 448 nm (standard) 488nm (GFP/YFP discrimination) A B C A B C 685LP, 710/50 505LP, 530/30 488/10 525LP, 542/27 505LP, 510/20 488/10 View and Download fortessa FTK5 instructions manual online.

It allows detection of Our manuals: Cytoflex Biovis manual or Cytoflex quick start Home News/Seminar Listings Directory Confessions of BD LSRFortessa™ Cellular Analyzer offers the ultimate in choice for cytometry flow, providing power, Each software package has its own reference manual, provided on the documentation CD. BD Biosciences recommends that first-time users of this instrument take. 25 Aug 2017 This video describes the process of manual and auto compensation in BD FACSDiva on the LSR II. Fortessa 4-15.

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If you are not familiar DIGITAL KEYPAD FTK1 Distributed by : Audio Visual Security 10 Sandersons Way Marton Blackpool FY4 4NB General Enquiries : sales.avs@adivision.co.uk www.audiovisualsecurity.org.uk BD LSRFortessa™ X-20 Cell Analyzer Technical Specifications The BD LSRFortessa™ X-20 cell analyzer puts the power of the BD LSR platform into a compact footprint. It can easily fit on the benchtop for more cost-effective space utilization. The instrument delivers the optimal sensitivity and resolution required for multicolor applications. The BD LSR Fortessa™ X-20 cell analyzer delivers high-performance multicolor analysis detecting up to 18 parameters simultaneously with using 5 lasers: UV (355 nm) Violet (405 nm) Blue (488 nm) Y-G (561 nm) Red (640 nm) Fortessa X20 Flow Cytometer Quick guide Operation of the 5 laser Fortessa X20 BD Webinar CS&T System BD Compensation Guide… BD FACSDiva Software Reference Manual.

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HTS Sampler Daily Operation Tutorial . BD Cytometer Setup and Tracking Application Guide The BD LSR II is the first and only air-cooled, four-laser benchtop flow cytometer with the ability to acquire up to 18 colors. At the heart of the BD LSR II is the new digital acquisition, allowing for additional parameters to be detected, and yielding more information per cell. BD Biosciences digital flow cytometry software provides 2010-7-12 · I don't have an LSR, but an AriaII so some of the specifics will be different, but the principles the same. First, BD has a DIVA software tutorial - you should have gotten a copy with the instrument.

The BD LSRFortessa™ X-20 cell analyzer delivers high-performance multicolor analysis with the most compact footprint in its class at just 30" x 29" (76.2 x 73.7 cm) and a height of 30" (76.2 cm). FORTESSA reserves the right to change the characteristics and dimensions of the equipment without prior warning.
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The instrument delivers the optimal sensitivity and resolution required for multicolor applications. The BD LSR Fortessa™ X-20 cell analyzer delivers high-performance multicolor analysis detecting up to 18 parameters simultaneously with using 5 lasers: UV (355 nm) Violet (405 nm) Blue (488 nm) Y-G (561 nm) Red (640 nm) Fortessa X20 Flow Cytometer Quick guide Operation of the 5 laser Fortessa X20 BD Webinar CS&T System BD Compensation Guide… BD FACSDiva Software Reference Manual.

• Becton Dickinson LSR II. Page 1. BD FACSDiva 6.1.2 – Tutorial. USUHS. Flow Cytometry Core Facility.
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Fortessa Door Handles are covered by a 10 Year Guarantee on mechanical parts. Our levers have been tested to pass DIN EN 1906 European Quality Standards, and have been tested for frequency of use. Essentially each door handle is guaranteed for 100,000 revolutions. The BD LSRFortessa X-20 cell analyzer is a benchtop digital flow cytometer equipped with a fixed-alignment flow cell that is gel-coupled to the collection optics. It has four spatially separated lasers - 405 nm, 488 nm, 561 nm and 640 nm. Configuration of Fortessa Laser Detector Band Pass Filter Dichoric Filter Color name available on the instrument Additional Color Channel in Fortessa_FTK5_Instructions_Rev01 2 There are 600 user codes (fobs or cards) and 1 programming code: Careful administration records will be required for future reference, please refer to last page of the instructions for an example sheet. Important Note: If a code is required then this has to be programmed with a fob or card To add fob or card Fortessa Grand City 18/10 Stainless Steel Flatware, 5 Piece Place Setting, Service for 1 , Silver - 4.5 out of 5 stars 17.

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BD High Throughput Sampler User’s Guide For the BD LSRFortessa I. Introduction The BD High Throughput Sampler (HTS) is a high-speed sampling device used on the 5-Laser LSRFortessa located in Rm. 6006F of the RIA Division. The HTS provides users the ability to acquire samples from 96-well or 384-well plates for efficient analysis with BD LSR Fortessa Standard Operation Protocol Refill Sheath Container 1. Make sure the flow cytometer is in STANDBY mode. 2. Disconnect the air line (green) and fluid line (Blue) from the sheath container.

E 123 4 5 6 789 0 1234 5678 90 E FT3328 FT3188 Technical characteristics • Total no. Of codes available: 302 - one supercode; - 300 relay codes. The total number of codes available can be distributed as desired between relay 1 and Crafthouse Informed design inspired by the streamlined aesthetic of Deco architecture.